Concerted modification of nucleotides at functional centers of the ribosome revealed by single-molecule RNA modification profiling.

ABSTRACT: Nucleotides in RNA and DNA are chemically modified by numerous enzymes that alter their function. Eukaryotic ribosomal RNA (rRNA) is modified at more than 100 locations, particularly at highly conserved and functionally important nucleotides. During ribosome biogenesis, modifications are added at various stages of assembly. The existence of differently modified classes of ribosomes in normal cells is unknown because no method exists to simultaneously evaluate the modification status at all sites within a single rRNA molecule. Using a combination of yeast genetics and nanopore direct RNA sequencing, we developed a reliable method to track the modification status of single rRNA molecules at 37 sites in 18 S rRNA and 73 sites in 25 S rRNA. We use our method to characterize patterns of modific

SUBMITTER: Bailey AD 

PROVIDER: S-EPMC9045821 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature