Plasma membrane H⁺-ATPases promote TORC1 activation in plant suspension cells.

ABSTRACT: The TORC1 (Target of Rapamycin Complex 1) kinase complex plays a pivotal role in controlling cell growth in probably all eukaryotic species. The signals and mechanisms regulating TORC1 have been intensely studied in mammals but those of fungi and plants are much less known. We have previously reported that the yeast plasma membrane H⁺-ATPase Pma1 promotes TORC1 activation when stimulated by cytosolic acidification or nutrient-uptake-coupled H⁺ influx. Furthermore, a homologous plant H⁺-ATPase can substitute for yeast Pma1 to promote this H⁺-elicited TORC1 activation. We here report that TORC1 activity in Nicotiana tabacum BY-2 cells is also strongly influenced by the activity of plasma membrane H⁺-ATPases. In particular, stimula

SUBMITTER: Primo C 

PROVIDER: S-EPMC9046228 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature