Multimodal imaging of synaptic vesicles with a single probe.

ABSTRACT: A complete understanding of synaptic-vesicle recycling requires the use of multiple microscopy methods to obtain complementary information. However, many currently available probes are limited to a specific microscopy modality, which necessitates the use of multiple probes and labeling paradigms. Given the complexity of vesicle populations and recycling pathways, having new single-vesicle probes that could be used for multiple microscopy techniques would complement existing sets of tools for studying vesicle function. Here, we present a probe based on the membrane-binding C2 domain of cytosolic phospholipase A₂ (cPLA₂) that fulfills this need. By conjugating the C2 domain with different detectable tags, we demonstrate that a single, modular probe can allow synaptic ve

SUBMITTER: An SJ 

PROVIDER: S-EPMC9046237 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature