Impact of disabled circadian clock on yellow fever mosquito Aedes aegypti fitness and behaviors.

ABSTRACT: Like other insects, Aedes aegypti displays strong daily patterns in host seeking and mating. Much of these behaviors are believed to be under the control of a circadian clock, an endogenous timekeeping mechanism relying on transcriptional/translational negative feedback loops that drive rhythmic physiology and behavior. To examine the connection between the circadian clock and various Ae. aegypti behaviors, we knocked out the core clock gene cycle using CRISPR/Cas9. We found that the rhythmic pattern and intensity of mRNA expression of seven circadian genes, including AeCyc^-/-, were altered across the day/night cycle as well as in constant darkness conditions. We further show that the mutant CYC protein is incapable of forming a dimer with CLK to stimulate per expression and tha

SUBMITTER: Shetty V 

PROVIDER: S-EPMC9046260 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature