Mutation in SF3B1 gene promotes formation of polyploid giant cells in Leukemia cells.

ABSTRACT: Giant cells with polyploidy, termed polyploid giant cells, have been observed during normal growth, development, and pathologic states, such as solid cancer progression and resistance to therapy. Functional studies of polyploidal giant cancer cells (PGCC) provided evidence that they arise when normal diploid cells are stressed, show stem cell-like properties, and give rise to tumors. In the present study, we report in K562 leukemia cell line that introduction of the hotspot K700E mutation in the gene SF3B1 using CRISPR/Cas9 method results in an increased frequency of multinucleated polyploid giant cells resistant to chemotherapeutic agent and serum starvation stress. These giant cells with higher ploidy are distinct from multinucleated megakaryocytes, are proliferative, and are characteriz

SUBMITTER: Mukherjee S 

PROVIDER: S-EPMC9046281 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature