A high-resolution route map reveals distinct stages of chondrocyte dedifferentiation for cartilage regeneration.

ABSTRACT: Articular cartilage damage is a universal health problem. Despite recent progress, chondrocyte dedifferentiation has severely compromised the clinical outcomes of cell-based cartilage regeneration. Loss-of-function changes are frequently observed in chondrocyte expansion and other pathological conditions, but the characteristics and intermediate molecular mechanisms remain unclear. In this study, we demonstrate a time-lapse atlas of chondrocyte dedifferentiation to provide molecular details and informative biomarkers associated with clinical chondrocyte evaluation. We performed various assays, such as single-cell RNA sequencing (scRNA-seq), live-cell metabolic assays, and assays for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq), to develop a biphasic dediffere

SUBMITTER: Chen Y 

PROVIDER: S-EPMC9046296 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature