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O mouse, where art thou? The Mouse Position Surveillance System (MoPSS)-an RFID-based tracking system.

ABSTRACT: Existing methods for analysis of home cage-based preference tests are either time-consuming, not suitable for group management, expensive, and/or based on proprietary equipment that is not freely available. To correct this, we developed an automated system for group-housed mice based on radio frequency identification: the Mouse Position Surveillance System (MoPSS). The system uses an Arduino microcontroller with compatible components; it is affordable and easy to rebuild for every laboratory because it uses free and open-source software and open-source hardware with the RFID readers as the only proprietary component. The MoPSS was validated using female C57BL/6J mice and manual video comparison. It proved to be accurate even for fast-moving mice (up to 100% accuracy after logical reconstruction), and is already implemented in several studies in our laboratory. Here, we provide the complete construction description as well as the validation data and the results of an example experiment. This tracking system will allow group-based preference testing with individually identified mice to be carried out in a convenient manner. This facilitation of preference tests creates the foundation for better housing conditions from the animals' perspective.

SUBMITTER: Habedank A

PROVIDER: S-EPMC9046340 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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O mouse, where art thou? The Mouse Position Surveillance System (MoPSS)-an RFID-based tracking system.

Habedank Anne A Urmersbach Birk B Kahnau Pia P Lewejohann Lars L

Behavior research methods 20210803 2

Existing methods for analysis of home cage-based preference tests are either time-consuming, not suitable for group management, expensive, and/or based on proprietary equipment that is not freely available. To correct this, we developed an automated system for group-housed mice based on radio frequency identification: the Mouse Position Surveillance System (MoPSS). The system uses an Arduino microcontroller with compatible components; it is affordable and easy to rebuild for every laboratory bec ...[more]

PMID: 34346041

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Project description:Radio frequency identification (RFID) has become a key technology in the logistics and management industry, thanks to distinctive features such as the low cost of RFID tags, and the easiness of the RFID tags' deployment and integration within the items to be tracked. In consequence, RFID plays a fundamental role in the so-called digital factory or 4.0 Industry, aiming to increase the level of automatization of industrial processes. In addition, RFID has also been found to be of great help in improving the tracking of patients, medicines, and medical assets in hospitals, where the digitalization of these operations improves their efficiency and safety. This contribution reviews the state-of-the-art of RFID for e-Health applications, describing the contributions to improve medical services and discussing the limitations. In particular, it has been found that a lot of effort has been put into software development, but in most of the cases a detailed study of the physical layer (that is, the characterization of the RFID signals within the area where the system is deployed) is not properly conducted. This contribution describes a basic RFID system for tracking and managing assets in hospitals, aiming to provide additional details about implementation aspects that must be considered to ensure proper functionality of the system. Although the scope of the RFID system described in this contribution is restricted to a small area of the hospital, the architecture is fully scalable to cover the needs of the different medical services in the hospital. Ultra high-frequency (UHF) RFID technology is selected over the most extended near-field communication (NFC) and high-frequency (HF) RFID technology to minimize hardware infrastructure. In particular, UHF RFID also makes the coverage/reading area conformation easier by using different kinds of antennas. Information is stored in a database, which is accessed from end-user mobile devices (tablets, smartphones) where the position and status of the assets to be tracked are displayed.

Dataset Information

O mouse, where art thou? The Mouse Position Surveillance System (MoPSS)-an RFID-based tracking system.

Publications

O mouse, where art thou? The Mouse Position Surveillance System (MoPSS)-an RFID-based tracking system.

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