Systematic evaluation of colorectal cancer organoid system by single-cell RNA-Seq analysis.

ABSTRACT:

Background

Patient-derived organoid culture is a powerful system for studying the molecular mechanisms of cancers, especially colorectal cancer (CRC), one of the most prevalent cancers worldwide. There are two main types of 3D culture methods for colonic cells, but the similarities and differences between gene expression patterns in different culture media remain largely unexplored.

Results

Here, we establish patient-derived organoids from colorectal cancer patients and perform single-cell RNA-Seq for pairwise samples from seven patients for both organoids and their corresponding tumor and normal tissues in vivo. We find that organoids derived from tumor tissues faithfully recapitulate the main gene expression signatures of cancer cells in vivo. On the other hand, organoids

SUBMITTER: Wang R 

PROVIDER: S-EPMC9047329 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature