Development of an auto-inducible expression system by nitrogen sources switching based on the nitrogen catabolite repression regulation.

ABSTRACT:

Background

The construction of protein expression systems is mainly focused on carbon catabolite repression and quorum-sensing systems. However, each of these regulatory modes has an inherent flaw, which is difficult to overcome. Organisms also prioritize using different nitrogen sources, which is called nitrogen catabolite repression. To date, few gene regulatory systems based on nitrogen catabolite repression have been reported.

Results

In this study, we constructed a nitrogen switching auto-inducible expression system (NSAES) based on nitrogen catabolite regulation and nitrogen utilization in Aspergillus nidulans. The P_niaD promoter that is highly induced by nitrate and inhibition by ammonia was used as the promoter. Glucuronidase was the reporter protein. Gluc

SUBMITTER: Yan Q 

PROVIDER: S-EPMC9047365 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature