Characterization and identification of cell death dynamics by quantitative phase imaging.

ABSTRACT:

Significance

Investigating cell death dynamics at the single-cell level plays an essential role in biological research. Quantitative phase imaging (QPI), a label-free method without adverse effects of exogenous labels, has been widely used to image many types of cells under various conditions. However, the dynamics of QPI features during cell death have not been thoroughly characterized.

Aim

We aim to develop a label-free technique to quantitatively characterize single-cell dynamics of cellular morphology and intracellular mass distribution of cells undergoing apoptosis and necrosis.

Approach

QPI was used to capture time-lapse phase images of apoptotic, necrotic, and normal cells. The dynamics of morphological and QPI features during cell death were fitted by a sigmoi

SUBMITTER: Hsieh HC 

PROVIDER: S-EPMC9047449 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature