Enzyme Purification Improves the Enzyme Loading, Self-Propulsion, and Endurance Performance of Micromotors.

ABSTRACT: Enzyme-powered micro- and nanomotors make use of biocatalysis to self-propel in aqueous media and hold immense promise for active and targeted drug delivery. Most (if not all) of these micro- and nanomotors described to date are fabricated using a commercially available enzyme, despite claims that some commercial preparations may not have a sufficiently high degree of purity for downstream applications. In this study, the purity of a commercial urease, an enzyme frequently used to power the motion of micro- and nanomotors, was evaluated and found to be impure. After separating the hexameric urease from the protein impurities by size-exclusion chromatography, the hexameric urease was subsequently characterized and used to functionalize hollow silica microcapsules. Micromotors loaded with pu

SUBMITTER: Valles M 

PROVIDER: S-EPMC9047656 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature