Improved Productivity of Streptomyces mobaraensis Transglutaminase by Regulating Zymogen Activation.

ABSTRACT: Streptomyces mobaraensis transglutaminase (TGase) is extracellularly expressed as a zymogen and then activated by TGase-activating protease (TAP). In this study, we reported the strategy for improving TGase production via the regulation of TAP activity in S. mobaraensis. First, we analyzed the effects of three inorganic nitrogen sources on TGase production. With 30 mM nitrogen content, the time to the peak of TGase activity induced by (NH₄)₂SO₄ or NH₄Cl was 72 h, 12 h earlier than that of the fermentation without adding NH₄ ⁺. SDS-PAGE analysis indicated that NH₄ ⁺ accelerated the TGase activation in S. mobaraensis. Then, we examined the effect of NH₄ ⁺

SUBMITTER: Yin X 

PROVIDER: S-EPMC9047793 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature