In vivo whole brain microvascular imaging in mice using transcranial 3D Ultrasound Localization Microscopy.

ABSTRACT:

Background

Non-invasive high-resolution imaging of the cerebral vascular anatomy and function is key for the study of intracranial aneurysms, stenosis, arteriovenous malformations, and stroke, but also neurological pathologies, such as degenerative diseases. Direct visualization of the microvascular networks in the whole brain remains however challenging in vivo.

Methods

In this work, we performed 3D ultrafast ultrasound localization microscopy (ULM) using a 2D ultrasound matrix array and mapped the whole-brain microvasculature and flow at microscopic resolution in C57Bl6 mice in vivo.

Findings

We demonstrated that the mouse brain vasculature can be imaged directly through the intact skull at a spatial resolution of 20 µm and over the whole brain depth and at high tem

SUBMITTER: Demeulenaere O 

PROVIDER: S-EPMC9048085 | biostudies-literature | 2022 May

REPOSITORIES: biostudies-literature