Angiopoietin-like 4 governs diurnal lipoprotein lipase activity in brown adipose tissue.

ABSTRACT:

Objective

Brown adipose tissue (BAT) burns fatty acids (FAs) to produce heat, and shows diurnal oscillation in glucose and triglyceride (TG)-derived FA-uptake, peaking around wakening. Here we aimed to gain insight in the diurnal regulation of metabolic BAT activity.

Methods

RNA-sequencing, chromatin immunoprecipitation (ChIP)-sequencing, and lipidomics analyses were performed on BAT samples of wild type C57BL/6J mice collected at 3-hour intervals throughout the day. Knockout and overexpression models were used to study causal relationships in diurnal lipid handling by BAT.

Results

We identified pronounced enrichment of oscillating genes involved in extracellular lipolysis in BAT, accompanied by oscillations of FA and monoacylglycerol content. This coincided with peak

SUBMITTER: van Eenige R 

PROVIDER: S-EPMC9048098 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature