Microfluidic platform for spatially segregated experimental evolution studies with E. coli.

ABSTRACT: Microdroplet emulsions allow investigators to build controllable microenvironments for applications in experimental evolution and synthetic ecology. We designed a microfluidic platform that uses highly homogenous microdroplets to enable these experiments. We also present a step-by-step protocol for the rapid production of highly homogeneous microdroplets suitable for experimental evolution. We also describe protocols for the propagation and serial passage of microbial populations across a range of selection schemes and potential spatial structures. For complete details on the use and execution of this protocol, please refer to Seo et al. (2021).

SUBMITTER: Seo S 

PROVIDER: S-EPMC9048157 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature