Metabolomics-Driven Identification of the Rate-Limiting Steps in 1-Propanol Production.

ABSTRACT: The concerted effort for bioproduction of higher alcohols and other commodity chemicals has yielded a consortium of metabolic engineering techniques to identify targets to enhance performance of engineered microbial strains. Here, we demonstrate the use of metabolomics as a tool to systematically identify targets for improved production phenotypes in Escherichia coli. Gas chromatography/mass spectrometry (GC/MS) and ion-pair LC-MS/MS were performed to investigate metabolic perturbations in various 1-propanol producing strains. Two initial strains were compared that differ in the expression of the citramalate and threonine pathways, which hold a synergistic relationship to maximize production yields. While this results in increased productivity, no change in titer was observed when t

SUBMITTER: Ohtake T 

PROVIDER: S-EPMC9048197 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature