Integrated bioinformatics analysis for the identification of hub genes and signaling pathways related to circANRIL.

ABSTRACT:

Background

Antisense noncoding RNA in the INK4 locus (ANRIL) is located on human chromosome 9p21, and modulation of ANRIL expression mediates susceptibility to some important human disease, including atherosclerosis (AS) and tumors, by affecting the cell cycle circANRIL and linear ANRIL are isoforms of ANRIL. However, it remains unclear whether these isoforms have distinct functions. In our research, we constructed a circANRIL overexpression plasmid, transfected it into HEK-293T cell line, and explored potential core genes and signaling pathways related to the important differential mechanisms between the circANRIL-overexpressing cell line and control cells through bioinformatics analysis.

Methods

Stable circANRIL-overexpressing (circANRIL-OE) HEK-293T cells and control cell

SUBMITTER: Qin Q 

PROVIDER: S-EPMC9048645 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature