High-Force Application by a Nanoscale DNA Force Spectrometer.

ABSTRACT: The ability to apply and measure high forces (>10 pN) on the nanometer scale is critical to the development of nanomedicine, molecular robotics, and the understanding of biological processes such as chromatin condensation, membrane deformation, and viral packaging. Established force spectroscopy techniques including optical traps, magnetic tweezers, and atomic force microscopy rely on micron-sized or larger handles to apply forces, limiting their applications within constrained geometries including cellular environments and nanofluidic devices. A promising alternative to these approaches is DNA-based molecular calipers. However, this approach is currently limited to forces on the scale of a few piconewtons. To study the force application capabilities of DNA devices, we implemented DNA o

SUBMITTER: Darcy M 

PROVIDER: S-EPMC9048690 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature