Plasma Amino Acids During 8 Weeks of Overfeeding: Relation to Diet Body Composition and Fat Cell Size in the PROOF Study.

ABSTRACT:

Objective

Different amounts of dietary protein during overfeeding produced similar fat gain but different amounts of gain in fat-free body mass. Protein and energy intake may have differential effects on amino acids during overfeeding.

Methods

Twenty-three healthy adult men and women were overfed by 40% for 8 weeks with 5%, 15%, or 25% protein diets. Plasma amino acids were measured by gas chromatography and mass spectrometry at baseline and week 8. Body composition was measured by dual-energy x-ray absorptiometry, fat cell size (FCS) from subcutaneous fat biopsies, and insulin resistance by euglycemic-hyperinsulinemic clamp.

Results

The following three amino acid patterns were seen: increasing concentration of five essential and three nonessential amino acids with in

SUBMITTER: Bray GA 

PROVIDER: S-EPMC9048866 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature