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Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water.

ABSTRACT: Laccase was stably immobilized on a cost effective and nanosized magnetic biochar (L-MBC) by adsorption, precipitation and crosslinking, and it was used for high performance BPA removal. A large amount of enzyme could be immobilized on the magnetic biochar with high activity (2.251 U per mg MBC), and the L-MBC could be magnetically separated from the aqueous solution in 20 seconds. The successful immobilization of laccase was also confirmed via FTIR, SEM, and EDS analyses. The L-MBC presented better storage and stability performances, pH tolerance and thermal stability than the free laccase. It was found that BPA with an initial concentration of 25 mg L^-1 could be thoroughly removed within 75 min, where BPA removal was attributed to enzymatic degradation and adsorption. In addition, the BPA removal efficiency by the L-MBC could be maintained above 85% even after seven cycles of repeated use. Due to high stability and efficient recyclability, the L-MBC-based biocatalyst has the potential to be a reliable method for treating BPA in environmental water sources.

SUBMITTER: Zhang Y

PROVIDER: S-EPMC9049069 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water.

Zhang Yu Y Piao Mingyue M He Lingzhi L Yao Lan L Piao Tiezhu T Liu Zairan Z Piao Yunxian Y

RSC advances 20200130 8

Laccase was stably immobilized on a cost effective and nanosized magnetic biochar (L-MBC) by adsorption, precipitation and crosslinking, and it was used for high performance BPA removal. A large amount of enzyme could be immobilized on the magnetic biochar with high activity (2.251 U per mg MBC), and the L-MBC could be magnetically separated from the aqueous solution in 20 seconds. The successful immobilization of laccase was also confirmed <i>via</i> FTIR, SEM, and EDS analyses. The L-MBC prese ...[more]

PMID: 35495269

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Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water.

Publications

Immobilization of laccase on magnetically separable biochar for highly efficient removal of bisphenol A in water.

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