Long-read and chromosome-scale assembly of the hexaploid wheat genome achieves high resolution for research and breeding.

ABSTRACT:

Background

The sequencing of the wheat (Triticum aestivum) genome has been a methodological challenge for many years owing to its large size (15.5 Gb), repeat content, and hexaploidy. Many initiatives aiming at obtaining a reference genome of cultivar Chinese Spring have been launched in the past years and it was achieved in 2018 as the result of a huge effort to combine short-read sequencing with many other resources. Reference-quality genome assemblies were then produced for other accessions, but the rapid evolution of sequencing technologies offers opportunities to reach high-quality standards at lower cost.

Results

Here, we report on an optimized procedure based on long reads produced on the Oxford Nanopore Technology PromethION device to assemble the genome of the Frenc

SUBMITTER: Aury JM 

PROVIDER: S-EPMC9049114 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature