A fluorescence immunoassay based on CdTe : Zn/ZnS quantum dots for the rapid detection of bacteria, taking Delftia tsuruhatensis CM'13 as an example.

ABSTRACT: A fluorescence immunoassay has been widely applied in different fields due to its high sensitivity, simple operations, and high accuracy. Quantum dots (QDs) are often selected as labels in a fluorescence immunoassay due to their high fluorescence, better stability, and biocompatibility. In this study, novel doped CdTe : Zn/ZnS QDs with stability and a high photoluminescence quantum yield (40.78%) were prepared by the water synthesis method and used as labels to conjugate with goat anti-rabbit IgG to establish a fluorescence immunoassay (FLISA) for bacteria compared to the traditional enzyme-linked immunosorbent assay (ELISA) based on the reaction between an antibody and an antigen. A good linear relationship between the fluorescence intensity and concentrations of D. tsuruhatensis C

SUBMITTER: Ruan Y 

PROVIDER: S-EPMC9049142 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature