Modeling rapid and selective capture of nNOS-PSD-95 uncouplers from Sanhuang Xiexin decoction by novel molecularly imprinted polymers based on metal-organic frameworks.

ABSTRACT: Novel and highly selective molecularly imprinted polymers based on the surface of metal-organic frameworks, NH₂-MIL-101(Cr) (MIL@MIP_S), were successfully fabricated to capture neuronal nitric oxide synthase-postsynaptic density protein-95 (nNOS-PSD-95) uncouplers from Sanhuang Xiexin Decoction (SXD) for stroke treatment. The resultant polymers were characterized by Fourier transform infrared spectroscopy, scanning electron microscopy, thermogravimetric analysis, and X-ray diffraction. The performance tests revealed that MIL@MIPs had a large binding capacity, fast kinetics, and excellent selectivity. Then the obtained polymers were satisfactorily applied to solid-phase extraction coupled with high-performance liquid chromatography to selectively capture nNOS-PSD-95 unc

SUBMITTER: Pan L 

PROVIDER: S-EPMC9049783 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature