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A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer.


ABSTRACT: A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies.

SUBMITTER: Lou UK 

PROVIDER: S-EPMC9049936 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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A simple and rapid colorimetric detection of serum lncRNA biomarkers for diagnosis of pancreatic cancer.

Lou Ut Kei UK   Wong Chi Hin CH   Chen Yangchao Y  

RSC advances 20200225 14


A colorimetric assay is developed for detection of lncRNA HOTTIP by one-step reverse transcription-loop-mediated isothermal amplification (RT-LAMP) coupled with positively-charged gold nanoparticles ((+)AuNP) for diagnosis of pancreatic cancer. This assay allows simple, rapid, and sensitive quantification of lncRNA down to 50 copies. ...[more]

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