Detection of four phenolic oestrogens by a novel electrochemical immunosensor based on a hexestrol monoclonal antibody.

ABSTRACT: Using a sandwich-type immunoassay, a novel electrochemical immunosensor based on the successful preparation of a hexestrol (HEX) monoclonal antibody was successfully constructed to detect four phenolic oestrogens: HEX, diethylstilboestrol (DES), dienestrol (DE) and bisphenol A (BPA). An innovative strategy to design the HEX monoclonal antibody/mercapto acetic acid (MACA)/nanogold immunosensor was developed by combining a nanosized effect, self-assembly and specific immune technology. The differential pulse voltammetry (DPV) response for four phenolic oestrogens was in the order BPA > DE > HEX > DES with detection limits of 0.037, 0.047, 0.052 and 0.060 ng mL^-1, respectively (S/N = 3). This immunosensor exhibits superior electrochemical properties with a wide linear range, low de

SUBMITTER: Zhao GZ 

PROVIDER: S-EPMC9050000 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature