Dataset Information

Toxicity and biochemical impact of methoxyfenozide/spinetoram mixture on susceptible and methoxyfenozide-selected strains of Spodoptera littoralis (Lepidoptera: Noctuidae).

ABSTRACT: Methoxyfenozide (M) is one of the selective insecticides used in integrated pest management (IPM) programs for lepidopteran pests. However, recent studies reported a development of M-resistance, which prompted us to look for alternatives. Here, we investigate the potency of a mixture of M with spinetoram (Sp) on M-resistant insects. In the laboratory, a selection pressure with M has carried out on Spodoptera littoralis (Lepidoptera: Noctuidae) strains. A dipping technique was used to evaluate the toxicity of a sublethal concentration of M and Sp. on S. littoralis larvae, and the same concentrations were used to assess the toxic impact of their combination on susceptible (SUS) and M-selected (MS) strains. The toxicity of M/Sp mixtures was computed using a combination index equation, and a potentiation effect was observed in the two tested strains. Synergism tests revealed that piperonyl butoxide had considerable synergistic effects on M toxicity in the MS strain. The results revealed that the M/Sp mixture's negative effect on both monooxygenases and esterases is most likely the cause of its potentiation effect on the SUS and MS strains. It was concluded that M/Sp mixtures are effective against M-resistant S. littoralis strains, so these can be used in IPM programs.

SUBMITTER: Ahmed FS

PROVIDER: S-EPMC9050723 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature

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Toxicity and biochemical impact of methoxyfenozide/spinetoram mixture on susceptible and methoxyfenozide-selected strains of Spodoptera littoralis (Lepidoptera: Noctuidae).

Ahmed Fatma S FS Helmy Yasser S YS Helmy Walid S WS

Scientific reports 20220428 1

Methoxyfenozide (M) is one of the selective insecticides used in integrated pest management (IPM) programs for lepidopteran pests. However, recent studies reported a development of M-resistance, which prompted us to look for alternatives. Here, we investigate the potency of a mixture of M with spinetoram (Sp) on M-resistant insects. In the laboratory, a selection pressure with M has carried out on Spodoptera littoralis (Lepidoptera: Noctuidae) strains. A dipping technique was used to evaluate th ...[more]

PMID: 35484385

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Project description:BackgroundThe evolution of insecticide resistance in Spodoptera frugiperda (Lepidoptera: Noctuidae) has resulted in large economic losses and disturbances to the environment and agroecosystems. Resistance to lufenuron, a chitin biosynthesis inhibitor insecticide, was recently documented in Brazilian populations of S. frugiperda. Thus, we utilized large-scale cDNA sequencing (RNA-Seq analysis) to compare the pattern of gene expression between lufenuron-resistant (LUF-R) and susceptible (LUF-S) S. larvae in an attempt to identify the molecular basis behind the resistance mechanism(s) of S. frugiperda to this insecticide.ResultsA transcriptome was assembled using approximately 19.6 million 100 bp-long single-end reads, which generated 18,506 transcripts with a N50 of 996 bp. A search against the NCBI non-redundant database generated 51.1% (9,457) functionally annotated transcripts. A large portion of the alignments were homologous to insects, with the majority (45%) being similar to sequences of Bombyx mori (Lepidoptera: Bombycidae). Moreover, 10% of the alignments were similar to sequences of various species of Spodoptera (Lepidoptera: Noctuidae), with 3% of them being similar to sequences of S. frugiperda. A comparative analysis of the gene expression between LUF-R and LUF-S S. frugiperda larvae identified 940 differentially expressed transcripts (p ≤ 0.05, t-test; fold change ≥ 4). Six of them were associated with cuticle metabolism. Of those, four were overexpressed in LUF-R larvae. The machinery involved with the detoxification process was represented by 35 differentially expressed transcripts; 24 of them belonging to P450 monooxygenases, four to glutathione-S-transferases, six to carboxylases and one to sulfotransferases. RNA-Seq analysis was validated for a number of selected candidate transcripts by using quantitative real time PCR (qPCR).ConclusionsThe gene expression profile of LUF-R larvae of S. frugiperda differs from LUF-S larvae. In general, gene expression is much higher in resistant larvae when compared to the susceptible ones, particularly for those genes involved with pathways for xenobiotic detoxification, mainly represented by P450 monooxygenases transcripts. Our data indicate that enzymes involved with the detoxification process, and mostly the P450, are one of the resistance mechanisms employed by the LUF-R S. frugiperda larvae against lufenuron.

Project description:Lipids and carbohydrates are the two primary energy sources for both animals and insects. Energy homeostasis is under strict control by the neuroendocrine system, and disruption of energy homeostasis leads to the development of various disorders, such as obesity, diabetes, fatty liver syndrome, and cardiac dysfunction. One critical factor in this respect is feeding habits and diet composition. Insects are good models to study the physiological and biochemical background of the effect of diet on energy homeostasis and related disorders; however, most studies are based on a single model species, Drosophila melanogaster. In the current study, we examined the effects of four different diets, high fat (HFD), high sugar (HSD), calcium-rich (CRD), and a plant-based (PBD) on energy homeostasis in younger (third instar) and older (fifth instar) larvae of the Egyptian cotton leafworm, Spodoptera littoralis (Lepidoptera: Noctuidae) in comparison to a regular artificial bean diet. Both HSD and HFD led to weight gain, while CRD had the opposite effect and PBD had no effect in fifth instar larvae and pupae. The pattern was the same for HSD and CRD in third instar larvae while a reduction in weight was detected with HFD and PBD. Larval development was shortest with the HSD, while HFD, CRD, and PBD led to retardation compared to the control. Triglyceride (TG) levels were higher with HFD, HSD, and PBD, with larger lipid droplet sizes, while CRD led to a reduction of TG levels and lipid droplet size. Trehalose levels were highest with HSD, while CRD led to a reduction at third instar larvae, and HFD and PBD had no effect. Fifth instar larvae had similar levels of trehalose with all diets. There was no difference in the expression of the genes encoding neuropeptides SpoliAKH and SpoliILP1-2 with different diets in third instar larvae, while all three genes were expressed primarily with HSD, and SpolisNPF was primarily expressed with HFD in fifth instar larvae. In summary, different diet treatments alter the development of insects, and energy and metabolic pathways through the regulation of peptide hormones.

Dataset Information

Toxicity and biochemical impact of methoxyfenozide/spinetoram mixture on susceptible and methoxyfenozide-selected strains of Spodoptera littoralis (Lepidoptera: Noctuidae).

Publications

Toxicity and biochemical impact of methoxyfenozide/spinetoram mixture on susceptible and methoxyfenozide-selected strains of Spodoptera littoralis (Lepidoptera: Noctuidae).

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