Pico-washing: simultaneous liquid addition and removal for continuous-flow washing of microdroplets.

ABSTRACT: Droplet microfluidics is based on a toolbox of several established unit operations, including droplet generation, incubation, mixing, pico-injection, and sorting. In the last two decades, the development of droplet microfluidic systems, which incorporate these multiple unit operations into a workflow, has demonstrated unique capabilities in fields ranging from single-cell transcriptomic analyses to materials optimization. One unit operation that is sorely underdeveloped in droplet microfluidics is washing, exchange of the fluid in a droplet with a different fluid. Here, we demonstrate what we name the "pico-washer," a unit operation capable of simultaneously adding fluid to and removing fluid from droplets in flow while requiring only a small footprint on a microfluidic chip. We describe t

SUBMITTER: Siedlik MJ 

PROVIDER: S-EPMC9050730 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature