Periodic formation of epithelial somites from human pluripotent stem cells.

ABSTRACT: During embryonic development, epithelial cell blocks called somites are periodically formed according to the segmentation clock, becoming the foundation for the segmental pattern of the vertebral column. The process of somitogenesis has recently been recapitulated with murine and human pluripotent stem cells. However, an in vitro model for human somitogenesis coupled with the segmentation clock and epithelialization is still missing. Here, we report the generation of human somitoids, organoids that periodically form pairs of epithelial somite-like structures. Somitoids display clear oscillations of the segmentation clock that coincide with the segmentation of the presomitic mesoderm. The resulting somites show anterior-posterior and apical-basal polarities. Matrigel is essential for epithe

SUBMITTER: Sanaki-Matsumiya M 

PROVIDER: S-EPMC9050736 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature