Decomposing a deterministic path to mesenchymal niche formation by two intersecting morphogen gradients.

ABSTRACT: Organ formation requires integrating signals to coordinate proliferation, specify cell fates, and shape tissue. Tracing these events and signals remains a challenge, as intermediate states across many critical transitions are unresolvable over real time and space. Here, we designed a unique computational approach to decompose a non-linear differentiation process into key components to resolve the signals and cell behaviors that drive a rapid transition, using the hair follicle dermal condensate as a model. Combining scRNA sequencing with genetic perturbation, we reveal that proliferative Dkk1+ progenitors transiently amplify to become quiescent dermal condensate cells by the mere spatiotemporal patterning of Wnt/β-catenin and SHH signaling gradients. Together, they deterministically coordi

SUBMITTER: Qu R 

PROVIDER: S-EPMC9050909 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature