Myeloid Cell PKM2 Deletion Enhances Efferocytosis and Reduces Atherosclerosis.

ABSTRACT:

Background

The glycolytic enzyme PKM2 (pyruvate kinase muscle 2) is upregulated in monocytes/macrophages of patients with atherosclerotic coronary artery disease. However, the role of cell type-specific PKM2 in the setting of atherosclerosis remains to be defined. We determined whether myeloid cell-specific PKM2 regulates efferocytosis and atherosclerosis.

Methods

We generated myeloid cell-specific PKM2^-/- mice on Ldlr (low-density lipoprotein receptor)-deficient background (PKM2^mye-KOLdlr^-/-). Controls were littermate PKM2^WTLdlr^-/- mice. Susceptibility to atherosclerosis was evaluated in whole aortae and cross sections of the aortic sinus in male and female mice fed a high-fat Western diet for 14 weeks, starting at 8 we

SUBMITTER: Doddapattar P 

PROVIDER: S-EPMC9050913 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature