Down-regulation of long non-coding RNAs in reproductive aging and analysis of the lncRNA-miRNA-mRNA networks in human cumulus cells.

ABSTRACT:

Purpose

Long non-coding RNAs (lncRNAs) control gene expression at multiple levels. By interacting with microRNAs (miRNAs), they regulate their mRNA targets creating dynamic regulatory networks involved in different cellular processes. Their role in follicle development and oocyte maturation has recently emerged. lncRNA deregulation has been found associated with different pathological conditions. In this study, we identified differentially expressed lncRNAs in cumulus cells (CCs) isolated from MII oocytes of advanced maternal age women and proposed ceRNA-networks involved in signaling pathways crucial in ovarian folliculogenesis and female germ cell maturation.

Methods

We performed a high-throughput analysis of the expression profile of 68 lncRNAs from CCs of aged and young

SUBMITTER: Caponnetto A 

PROVIDER: S-EPMC9050988 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature