Dataset Information

Synthesis and antibacterial activity of iron manganite (FeMnO₃) particles against the environmental bacterium Bacillus subtilis.

ABSTRACT: Nanocrystalline iron manganite powder was synthesized using the sol-gel combustion process, with glycine as fuel. It was further calcined at 900 °C for 8 h, resulting in the formation of a loose cubic FeMnO₃ powder with a small specific surface area, net-like structure and plate-like particles as confirmed by XRD, N₂ physisorption, FESEM and TEM analyses. The metal ion release was studied by ICP-OES and showed that less than 10 ppb of Fe or Mn ions were released by leaching in water, but 0.36 ppm Fe and 3.69 ppm Mn was found in LB (Luria-Bertani) bacterial medium. The generation of reactive oxygen species (ROS) was monitored in distilled water and bacterial medium and showed that FeMnO₃ particles do not generate O₂˙^- ions with or without UV irradiation, but synthesize H₂O₂ and show an antioxidative effect. Besides the higher stability of FeMnO₃ particles in aqueous solution they showed an inhibitory effect on Bacillus subtilis growth in LB medium even at low concentrations (0.01 mg ml^-1), but not in BHI medium even at 1 mg ml^-1. This study points out that the mechanism of antibacterial action of engineered metal oxides needs continued investigation and specific experimental controls.

SUBMITTER: Vasiljevic ZZ

PROVIDER: S-EPMC9051578 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Publications

Synthesis and antibacterial activity of iron manganite (FeMnO3) particles against the environmental bacterium Bacillus subtilis.

Vasiljevic Zorka Z ZZ Dojcinovic Milena P MP Krstic Jugoslav B JB Ribic Vesna V Tadic Nenad B NB Ognjanovic Milos M Auger Sandrine S Vidic Jasmina J Nikolic Maria Vesna MV

RSC advances 20200406 23

Nanocrystalline iron manganite powder was synthesized using the sol-gel combustion process, with glycine as fuel. It was further calcined at 900 °C for 8 h, resulting in the formation of a loose cubic FeMnO3 powder with a small specific surface area, net-like structure and plate-like particles as confirmed by XRD, N2 physisorption, FESEM and TEM analyses. The metal ion release was studied by ICP-OES and showed that less than 10 ppb of Fe or Mn ions were released by leaching ...[more]

PMID: 35492976

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Project description:Iron (Fe) is one of the most important micronutrients for most life forms on earth. While abundant in soil, Fe bioavailability in oxic soil is very low. Under environmental conditions, bacteria need to acquire sufficient Fe to sustain growth while limiting the energy cost of siderophore synthesis. Biofilm formation might mitigate this Fe stress, since it was shown to accumulate Fe in certain Gram-negative bacteria and that this Fe could be mobilized for uptake. However, it is still unclear if, and to what extent, the amount of Fe accumulated in the biofilm can sustain growth and if the mobilization of this local Fe pool is modulated by the availability of environmental Fe (i.e., Fe outside the biofilm matrix). Here, we use a nondomesticated strain of the ubiquitous biofilm-forming soil bacterium Bacillus subtilis and stable Fe isotopes to precisely evaluate the origin of Fe during growth in the presence of tannic acid and hydroxides, used as proxies for different environmental conditions. We report that this B. subtilis strain can accumulate a large quantity of Fe in the biofilm, largely exceeding Fe associated with cells. We also report that only a fraction of biofilm-bound Fe is available for uptake in the absence of other sources of Fe in the vicinity of the biofilm. We observed that the availability of environmental Fe modulates the usage of this pool of biofilm-bound Fe. Finally, our data suggest that consumption of biofilm-bound Fe relates to the efficacy of B. subtilis to transport Fe from the environment to the biofilm, possibly through siderophores.IMPORTANCE Recent pieces of evidence suggest that Fe bound to the biofilm could assume at least two important functions, a local source of Fe for uptake and a support to extracellular metabolism, such as extracellular electron transfer. Our results show that B. subtilis can use biofilm-bound Fe for uptake only if it does not compromise Fe homeostasis of the biofilm, i.e., maintains a minimum Fe concentration in the biofilm for extracellular purposes. We propose a theoretical framework based on our results and recent literature to explain how B. subtilis manages biofilm-bound Fe and Fe uptake in response to environmental Fe availability. These results provide important insights into the management of biofilm-bound and environmental Fe by B. subtilis in response to Fe stress.

Dataset Information

Synthesis and antibacterial activity of iron manganite (FeMnO₃) particles against the environmental bacterium Bacillus subtilis.

Publications

Synthesis and antibacterial activity of iron manganite (FeMnO<sub>3</sub>) particles against the environmental bacterium <i>Bacillus subtilis</i>.

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