Design of a gold nanoprobe for the detection of Pseudomonas aeruginosa elastase gene (lasB).

ABSTRACT: Nosocomial infections are one of the major health problems that increase mortality. Pseudomonas aeruginosa is one of the important causes of nosocomial infections. This bacterium has a gene called the lasB gene, the product of which is a zinc-dependent metalloprotease. This gene plays a central role in the pathogenesis of Pseudomonas spp. This pathogen is highly toxic and destroys tissues and also has a moderating effect on the immune system; on the other hand, it initiates the intracellular pathway of biofilm growth. Although there are methods such as molecular methods for identifying the lasB gene, due to the high cost and the need for specialized personnel, it is necessary to replace them with an appropriate method. In this study, a gold nanoparticle-based DNA diagnostic s

SUBMITTER: Farhangi A 

PROVIDER: S-EPMC9051652 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature