Time Evolution of the Millisecond Allosteric Activation of Imidazole Glycerol Phosphate Synthase.

ABSTRACT: Deciphering the molecular mechanisms of enzymatic allosteric regulation requires the structural characterization of functional states and also their time evolution toward the formation of the allosterically activated ternary complex. The transient nature and usually slow millisecond time scale interconversion between these functional states hamper their experimental and computational characterization. Here, we combine extensive molecular dynamics simulations, enhanced sampling techniques, and dynamical networks to describe the allosteric activation of imidazole glycerol phosphate synthase (IGPS) from the substrate-free form to the active ternary complex. IGPS is a heterodimeric bienzyme complex whose HisH subunit is responsible for hydrolyzing glutamine and delivering ammonia for the cycla

SUBMITTER: Calvo-Tusell C 

PROVIDER: S-EPMC9052757 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature