Spatiotemporal monitoring of intracellular metabolic dynamics by resonance Raman microscopy with isotope labeling.

ABSTRACT: Cellular metabolites are valuable in a diverse range of applications. For example, the unicellular green alga Haematococcus lacustris produces as a secondary metabolite the carotenoid pigment astaxanthin (AXT), which is widely used in nutraceutical, cosmetic, and food industries due to its strong antioxidant activity. In order to enhance the productivity of H. lacustris, spatial and temporal understanding of its metabolic dynamics is essential. Here we show spatiotemporal monitoring of AXT production in H. lacustris cells by resonance Raman microscopy combined with stable isotope labeling. Specifically, we incorporated carbon dioxide (¹³CO₂) labeled with a stable isotope (¹³C) into H. lacustris cells through carbon fixation and t

SUBMITTER: Yonamine Y 

PROVIDER: S-EPMC9053077 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature