Heat and pressure-resistant room temperature irreversible sealing of hybrid PDMS-thermoplastic microfluidic devices via carbon-nitrogen covalent bonding and its application in a continuous-flow polymerase chain reaction.

ABSTRACT: In this study, we have introduced a facile room-temperature strategy for irreversibly sealing polydimethylsiloxane (PDMS) elastomers to various thermoplastics using (3-aminopropyl)triethoxysilane (APTES) and [2-(3,4-epoxycyclohexyl)ethyl]trimethoxysilane (ECTMS), which can resist heat and pressure after sealing due to the high chemical reactivity of the used chemicals. An irreversible chemical bond was realized at RT within 30 min through the initial activation of PDMS and thermoplastics using oxygen plasma, followed by surface modification using amino- and epoxy-based silane coupling reagents on either side of the substrates and then conformally contacting each other. Surface characterizations were performed using contact angle measurements, fluorescence measurements, X-ray photoelectron

SUBMITTER: Sivakumar R 

PROVIDER: S-EPMC9053085 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature