A rapid antibody screening haemagglutination test for predicting immunity to SARS-CoV-2 variants of concern.

ABSTRACT:

Background

Evaluation of susceptibility to emerging SARS-CoV-2 variants of concern (VOC) requires rapid screening tests for neutralising antibodies which provide protection.

Methods

Firstly, we developed a receptor-binding domain-specific haemagglutination test (HAT) to Wuhan and VOC (alpha, beta, gamma and delta) and compared to pseudotype, microneutralisation and virus neutralisation assays in 835 convalescent sera. Secondly, we investigated the antibody response using the HAT after two doses of mRNA (BNT162b2) vaccination. Sera were collected at baseline, three weeks after the first and second vaccinations from older (80-99 years, n = 89) and younger adults (23-77 years, n = 310) and compared to convalescent sera from naturally infected individuals (1-89 yea

SUBMITTER: Ertesvag NU 

PROVIDER: S-EPMC9053181 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature