A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies.

ABSTRACT:

Background

Neutralizing antibodies (NAbs) prevent pathogens from infecting host cells. Detection of SARS-CoV-2 NAbs is critical to evaluate herd immunity and monitor vaccine efficacy against SARS-CoV-2, the virus that causes COVID-19. All currently available NAb tests are lab-based and time-intensive.

Method

We develop a 10 min cellulose pull-down test to detect NAbs against SARS-CoV-2 from human plasma. The test evaluates the ability of antibodies to disrupt ACE2 receptor-RBD complex formation. The simple, portable, and rapid testing process relies on two key technologies: (i) the vertical-flow paper-based assay format and (ii) the rapid interaction of cellulose binding domain to cellulose paper.

Results

Here we show the construction of a cellulose-based vertical-flo

SUBMITTER: Kongsuphol P 

PROVIDER: S-EPMC9053278 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature