An inkjet-printed polysaccharide matrix for on-chip sample preparation in point-of-care cell counting chambers.

ABSTRACT: On-chip sample preparation in self-contained microfluidic devices is a key element to realize simple, low-cost, yet reliable in vitro diagnostics that can be carried out at the point-of-care (POC) with minimal training requirements by unskilled users. To address this largely unmet POC medical need, we have developed an optimized polysaccharide matrix containing the reagents which substantially improves our fully printed POC CD4 counting chambers for the monitoring of HIV patients. The simply designed counting chambers allow for capillary-driven filling with unprocessed whole blood. We carefully tailored a gellan/trehalose matrix for deposition by inkjet printing, which preserves the viability of immunostains during a shelf life of at least 3 months and enables controlled antibody re

SUBMITTER: Zhang X 

PROVIDER: S-EPMC9053629 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature