A multi-scale map of cell structure fusing protein images and interactions.

ABSTRACT: The cell is a multi-scale structure with modular organization across at least four orders of magnitude¹. Two central approaches for mapping this structure-protein fluorescent imaging and protein biophysical association-each generate extensive datasets, but of distinct qualities and resolutions that are typically treated separately^2,3. Here we integrate immunofluorescence images in the Human Protein Atlas⁴ with affinity purifications in BioPlex⁵ to create a unified hierarchical map of human cell architecture. Integration is achieved by configuring each approach as a general measure of protein distance, then calibrating the two measures using machine learning. The map, known as the multi-scale integrated cell (MuSIC 1.0), resolves 69 subcellular sy

SUBMITTER: Qin Y 

PROVIDER: S-EPMC9053732 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature