Cytotoxicity and degradation product identification of thermally treated ceftiofur.

ABSTRACT: Ceftiofur (CEF) is a cephalosporin antibiotic and is a commonly used drug in animal food production. As a heat-labile compound, the residual CEF toxicity after thermal treatment has rarely been reported. This study was to investigate the potential toxicity of thermally treated CEF and determine the toxic components. By cytotoxicity tests and liquid chromatography-mass spectrometry (LC-MS) assays, the cytotoxicity of the thermally treated CEF (TTC) and the components of TTC was identified, respectively. Our results showed that TTC exhibited significantly increased toxicity compared with CEF towards LO2 cells by inducing apoptosis. Through LC-MS assays, we identified that the toxic compound of TTC was CEF-aldehyde (CEF-1). The IC₅₀ value of CEF-1 on LO2 cells treated for 24 h was

SUBMITTER: Zhang H 

PROVIDER: S-EPMC9053768 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature