Fast automated processing of AFM PeakForce curves to evaluate spatially resolved Young modulus and stiffness of turgescent cells.

ABSTRACT: Atomic Force Microscopy (AFM) is a powerful technique for the measurement of mechanical properties of individual cells in two (x × y) or three (x × y × time) dimensions. The instrumental progress makes it currently possible to generate a large amount of data in a relatively short time, which is particularly true for AFM operating in so-called PeakForce tapping mode (Bruker corporation). The latter corresponds to an AFM probe that periodically hits the sample surface while the pico-newton level interaction force is recorded from cantilever deflection. The method provides unprecedented high-resolution (a few tens of nm) imaging of the mechanical features of soft biological samples (e.g. bacteria, yeasts) and of hard abiotic surfaces (e.g. minerals).

SUBMITTER: Offroy M 

PROVIDER: S-EPMC9054095 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature