Estimation of a stronger heparin binding locus in fibronectin domain III¹⁴ using thermodynamics and molecular dynamics.

ABSTRACT: The HEP II (Heparin-binding site II) region of fibronectin (FN) containing domain III¹⁴ plays a crucial role in cell adhesion and migration through heparin-binding on the cell surface. There are two such fibronectin heparin interacting peptide (FHIP I and FHIP II) sequences present in HEP II. However, the molecular principles by which these sites orchestrate heparin-binding processes are poorly understood. Such knowledge would have great implications in the therapeutic targeting of FN. With this aim, we have explored the binding studies of FHIP I and FHIP II with heparin using various biophysical methods. A fluorescence melting study specifically revealed the preference of heparin for domain III in FN, indicating the key contribution of FHIP I and FHIP II in heparin binding. In

SUBMITTER: Gupta S 

PROVIDER: S-EPMC9054198 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature