Confocal Microscope-Based Laser Ablation and Regeneration Assay in Zebrafish Interneuromast Cells.

ABSTRACT: Hair cells are mechanosensory cells that mediate the sense of hearing. These cells do not regenerate after damage in humans, but they are naturally replenished in non-mammalian vertebrates such as zebrafish. The zebrafish lateral line system is a useful model for characterizing sensory hair cell regeneration. The lateral line is comprised of hair cell-containing organs called neuromasts, which are linked together by a string of interneuromast cells (INMCs). INMCs act as progenitor cells that give rise to new neuromasts during development. INMCs can repair gaps in the lateral line system created by cell death. A method is described here for selective INMC ablation using a conventional laser-scanning confocal microscope and transgenic fish that express green fluorescent protein in INMCs. Tim

SUBMITTER: Volpe BA 

PROVIDER: S-EPMC9054354 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature