Insights into the structure and function of the human organic anion transporter 1 in lipid bilayer membranes.

ABSTRACT: The human SLC22A6/OAT1 plays an important role in the elimination of a broad range of endogenous substances and xenobiotics thus attracting attention from the pharmacological community. Furthermore, OAT1 is also involved in key physiological events such as the remote inter-organ communication. Despite its significance, the knowledge about hOAT1 structure and the transport mechanism at the atomic level remains fragmented owing to the lack of resolved structures. By means of protein-threading modeling refined by μs-scaled Molecular Dynamics simulations, the present study provides the first robust model of hOAT1 in outward-facing conformation. Taking advantage of the AlphaFold 2 predicted structure of hOAT1 in inward-facing conformation, we here provide the essential structural and functional

SUBMITTER: Janaszkiewicz A 

PROVIDER: S-EPMC9054760 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature