Computational profiling of hiPSC-derived heart organoids reveals chamber defects associated with NKX2-5 deficiency.

ABSTRACT: Heart organoids have the potential to generate primary heart-like anatomical structures and hold great promise as in vitro models for cardiac disease. However, their properties have not yet been fully studied, which hinders their wide spread application. Here we report the development of differentiation systems for ventricular and atrial heart organoids, enabling the study of heart diseases with chamber defects. We show that our systems generate chamber-specific organoids comprising of the major cardiac cell types, and we use single cell RNA sequencing together with sample multiplexing to characterize the cells we generate. To that end, we developed a machine learning label transfer approach leveraging cell type, chamber, and laterality annotations available for primary human fetal heart c

SUBMITTER: Feng W 

PROVIDER: S-EPMC9054831 | biostudies-literature | 2022 Apr

REPOSITORIES: biostudies-literature