Development of a nanobody tagged with streptavidin-binding peptide and its application in a Luminex fluoroimmunoassay for alpha fetal protein in serum.

ABSTRACT: Sensitive and accurate detection of disease-related biomarkers can promote the early screening and diagnosis of cancers for improving the prognosis and survival of patients. Herein alpha fetal protein (AFP) was selected as the model macromolecule antigen and we developed AFP-specific alpaca nanobodies (Nbs) from an immunized phage-displayed Nb library. Then Nbs tagged with streptavidin-binding peptide (Nb-SBP) were constructed and used to develop an Nb-SBP-mediated fluoroimmunoassay based on the Luminex-200 system (NS-LFIA). Based on the optimal experimental conditions, the NS-LFIA has a limit of detection of 0.237 ng mL^-1 with a linear detection range of 0.49-125 ng mL^-1. The average recovery rate and relative standard derivation were in the range of 98.2-110% and 2.

SUBMITTER: Chen Q 

PROVIDER: S-EPMC9054930 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature