Study of β-lactam-based drug interaction with albumin protein using optical, sensing, and docking methods.

ABSTRACT: The quality and strength of drug and albumin interaction affecting the drug-free concentration and physiological activity are important issues in pharmacokinetic research. In the present study, not only did we evaluate the binding strength of ceftriaxone and ceftizoxime to bovine serum albumin (BSA), but we also investigated the kinetic and thermodynamic parameters including KD, KA, ΔS, and ΔH. We applied in vitro optical fluorescence spectroscopy and surface plasmon resonance (SPR) sensing approaches as well as molecular docking analyses. The kinetic and thermodynamic investigations were done using different concentrations of drugs at three temperatures. Thermodynamic parameters visibly demonstrated that the binding was an exothermic and spontaneous process. The obtained negative values o

SUBMITTER: Monirinasab H 

PROVIDER: S-EPMC9054962 | biostudies-literature | 2022 Jun

REPOSITORIES: biostudies-literature