CdTe QD-based inhibition and reactivation assay of acetylcholinesterase for the detection of organophosphorus pesticides.

ABSTRACT: An enzyme immobilized glutathione (GSH)-capped CdTe quantum dot (QD)-based fluorescence assay has been developed for monitoring organophosphate pesticides. In principle, GSH-capped CdTe QDs exhibit higher sensitivity towards H₂O₂ produced from the active enzymatic reaction of acetylcholinesterase (AChE) and choline oxidase (CHOx), which results in the fluorescence (FL) "turn-off" of the GSH-capped CdTe QDs. A "turn-on" FL of the CdTe QDs at 520 nm was recovered in the presence of organophosphate (OP). The FL changes of the GSH-capped CdTe QD/AChE/CHOx biosensor reasonably correspond to the amount of OP pesticides. The detection limit of the CdTe/AChE/CHOx biosensor towards paraoxon, dichlorvos, malathion and triazophos was 1.62 × 10^-15 M, 75.3 × 10^-15<

SUBMITTER: Korram J 

PROVIDER: S-EPMC9055098 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature