A polycarboxylic chelating ligand for efficient resin purification of His-tagged proteins expressed in mammalian systems.

ABSTRACT: We describe the synthesis of a novel polyamino polycarboxylic ligand, its ability to coordinate metal-ions and attachment to a solid support designed for protein purification through Immobilised Metal-ion Affinity Chromatography (IMAC). The resin was found to be highly efficient for purification of His-tagged HCV E2 glycoproteins expressed in 293T mammalian cells.

SUBMITTER: Popescu CC 

PROVIDER: S-EPMC9055129 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature